expression of P53 in non small cell lung cancer

Despite major advances in cancer treatment in the past two decades, the prognosis of patients with lung cancer has improved only minimally. Although tumor stage is the most significant prognostic factor, the variation in survival within staging groups requires information about additional factors influencing the outcome. Among several genetic aberrations that have been implicated in lung cancer, mutations in the p53 gene are the most common. The aim of this study was to evaluate the expression of p53 in non-small cell lung cancer. To correlate the relation of p53 with some clinico-pathological parameters. And to compare the results with that of others. Tumor tissues from 52 patients with non small cell lung cancer [NSCLC] were assessed by immune-histochemistry for the expression of p53. The immunohistochemical study was performed on formalin-fixed, paraffin-embedded sections using LSAB immune-peroxidase method. Thirty nine [75%] of 52 patients revealed aberrant immunostaining for p53. No significant relation was observed between the p53 and patient age [P=0.596]. A significant association [P=0.048] was found between the p53 and the sex of the patient with higher expression in males and the p53 was significantly [P=0.0345] associated with histopathological type of tumor. Comparing p53 expression with grade resulted in a strong positive correlation [P= 0.0002]. The percentage of p53-positive tumors progressively increased from [2.56%] in well differentiation to [53.85%] in poorly differentiated tumor. P53 was significantly association with sex, grade and histological type, the detection of p53 may be important marker to predict the prognosis of the patients with NSCLC and for stratifying these patients into more accurate prognostic group.

Ki-67[MIB-1] and progesterone receptor in meningioma: an immunohistochemical study

In meningioma the tumor grade, subtype, and extent of surgical resection are strong prognostic factors, the growth of this tumor is still unpredictable, and additional prognostic markers are needed. Many studies have shown that the detection of the proliferative potential of meningioma by Ki-67 [MIB-l] and the detection of the progesterone receptor might predict the natural history of tumor and patient survival. To evaluate the proliferative fraction Ki-67 [MIB-l] and progesterone receptor in meningioma, To correlate the expression of these two markers with various clinico- pathological parameters, To compare these results with other studies. This is a prospective study, in which 50 cases of meningioma were collected in Nineveh province. These cases were examined immunohistochemically. The results of these two markers were correlated with various clinico- pathological parameters. The mean age of all cases was 46 years. There were 16 male and 34 female patients. According to the W.H.O 2000 classification, there were 84% grade l, 10% were grade II and 6% were grade III. Only 14% of the patients had a history of recurrence. Progesterone receptor was positive in 72% of all cases. Significant relation was observed between the progesterone receptor and the patients age .It was significantly higher in female than male. A significant relationship was found between progesterone receptor with grade and histological types. Thirty three out of 43 cases without recurrence had positive progesterone receptor. The mean +/- Standard deviation [SD] of Ki-67 Labeling Index [Ki-67 LI] was 2.7 +/- 4.2%. No significant relation was found between the Ki-67 LI and patients age. The male patients had a higher Ki-67 Ll than that of the female. Significant relation was found between Ki-67 Ll and the grade of meningioma. The mean of Ki-67 LI in the recurrent cases was higher than in non recurrent cases. An inverse relationship was found between Ki-67 LI and progesterone receptor. Progesterone receptor showed a significant relation with the age and histological types. Ki-67 LI had a direct significant association with the grade and the recurrent cases, while inverse relation was observed between the progesterone receptor with the grade and the recurrent cases. An inverse relationship was observed between the progesterone receptor and Ki-67 LI